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Retraction of rod photoreceptor axon terminals begins during the first 7 hours in culture. (A) Representative isolated rod photoreceptor in culture after 1 and 7 hours. Axon length was measured from the base of the cell body to the tip of the axon terminal, shown with red bars. N, nucleus; E, ellipsoid. Scale bar: 10 μm. (B) Mean length of axons was reduced by approximately 40% over 7 hours. n = 3 animals, 6 cultures, 59 rod cells, ∼30 cells per group; ***P < 0.001; Student's t-test.
