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. 2015 Dec 11;56(13):7847–7858. doi: 10.1167/iovs.15-17278

Figure 6.

Figure 6

Retraction of the axon is reduced by inhibiting the LIMK pathway. (A–C) Inhibiting ROCK or Pak, two different LIMK regulators, or inhibiting LIMK directly, with Y27632 (Y27), IPA-3, or BMS-5, respectively, reduced axonal retraction in a dose-dependent manner. (D) Concomitant inhibition of ROCK and Pak with 10 μM Y27632 and 1 μM IPA-3 resulted in significantly more effect on axonal retraction than either treatment alone. % Length reduction = (L1 − L7) ÷ L1 × 100%; L1, axon length in 1-hour culture; L7, axon length in 7-hour culture. All data were normalized to control (DMSO) group, which is set at 100%. (A) n = 9 animals, 22 cultures, 411 rod cells, ∼100 cells per group. (B) n = 7 animals, 26 cultures, 483 rod cells, ∼80 cells per group. (C) n = 5 animals, 34 cultures, 430 rod cells, ∼86 cells per group. (D) n = 9 animals, 40 cultures, 502 rod cells, ∼126 cells per group. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001; post hoc (Tukey's test), a: Y27 10 vs. 100 μM, a < 0.05; b1–b3: IPA3 0.3 vs. 1, 3, or 10 μM, respectively, b1 < 0.01, b2 < 0.001, b3 < 0.001; c1, c2: BMS-5 1 vs. 10 or 30 μM, respectively, c1 < 0.05, c2 < 0.01.