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. Author manuscript; available in PMC: 2015 Dec 17.
Published in final edited form as: Clin Cancer Res. 2015 May 14;21(16):3783–3793. doi: 10.1158/1078-0432.CCR-14-2829

Figure 1.

Figure 1

B[a]P/B[a]PDE downregulates PHLPP2 protein expression in vitro and in vivo. A and B, Beas-2B cells were exposed to 1 μmol/L of B[a]PDE for the times indicated (A), or various concentrations of B[a]PDE for 12 h (B); C, Beas-2B cells were treated with either arsenite, B[a]P, or B[a]PDE for 12 hours; D, A549 cells were treated with either B[a]P or B[a]PDE for 12 hours; E, A549 cells were exposed to the various doses of B[a]PDE for 12 hours; whole-cell extracts from each of above experiments were subjected to Western blotting for the determination of protein expression as indicated. Fand G, IHC-P was carried out to evaluate PHLPP2 expression in lung tissues from mice exposed to B[a]P for 180 days and the optical density was calculated as described in Materials and Methods section. Normal lung epithelial cells and pulmonary malignant epithelial cells were indicated in arrows. H and I, PHLPP2 expression in lung tissues from 54 pairs of human primary lung cancer was evaluated by IHC-P and the optical density was calculated as described in Materials and Methods. Arrows, normal lung epithelial cells and pulmonary malignant epithelial cells.