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. 2015 Oct 13;34(22):2758–2774. doi: 10.15252/embj.201591458

Figure 2. Nucleolar RNA is enriched in specific intron‐encoded aluRNAs.

Figure 2

  1. Left: Scheme illustrating the origin of forward or reverse intronic aluRNA and processing into left arm aluRNAs (aluRNAL) and right arm aluRNAs (aluRNAR). Right: Heatmaps of read density of forward and reverse intronic Alu RNA species present in the total RNA sample.
  2. Top: Heatmaps of read density of intronic Alu repeats in the nucleolar RNA fraction. For each aluRNA variant, the percentage over all transcribed intronic Alu repeats is indicated. Bottom: Ratio of nucleolar versus total RNA from higher (red) to lower (green) read density.
  3. View of nucleolus‐enriched Alu elements exemplified at two genomic loci. Mapping of normalized reads from total (red), nucleoplasmic (orange) and nucleolar (blue) RNA to intron #5 of DCP1A and intron #22 of UBE4B.
  4. Heatmaps of total RNA read density ratios between untreated (control) and α‐amanitin‐ or ML‐60218‐treated HeLa cells.
See also Appendix Figs S3 and S4, Appendix Tables S2 and S3.