Movie 2.
Growth of SGN processes in an E15 cochlear explant. Time-lapse movie of a suspended organotypic culture of an E15 Bhlhb5-cre; AI14 cochlea imaged after 12 h in culture. In contrast to what happens in typical 2D explants, processes remain within the cochlea and interact with cells in the organ of Corti (white wedges), including their natural synaptic partners. A magnified region from this preparation (rectangle) is RGB coded by depth to illustrate preservation of the dynamic 3D environment in live tissue. Time, hh:mm. Scale bar, 50 μm.