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. 2015 Dec 14;10(12):e0145143. doi: 10.1371/journal.pone.0145143

Fig 4. Antibacterial activity of gels.

Fig 4

Metabolic activity of bacterial supernatant after 24h (A). « Plastic » corresponds to a negative control, i.e. bacteria in medium on the 96-well plate, without any gel. « Medium » corresponds to culture media in the 96-well plate without any bacteria or gel. « Antibiotic » corresponds to the positive control with bacteria in medium with two standard antibiotics (tetracyclin and cefotoxim) as supplements. The asterisk (*) denotes a statistical difference between the metabolic activity of P. gingivalis found in the supernatants of AC and AC-CTL gels, (#) indicates a statistical difference between the metabolic activity of P. gingivalis found in the supernatant of AC/PlubisSH and AC/PlubisSH-CTL (p < 0.05). Inhibition of colonies forming units (CFU) of the supernatants after 24h of seeding (B and C). These supernatants were previously removed from the gels respectively after 5h (B) and 24h (C) of seeding. The control used in these figures (corresponding to 100% CFU) corresponds to colonies on agar plates obtained from supernatant of AC gel without CTL (Tissue Culture Polystyrene was not used as control because it leads to an homogenous growth of bacteria without colonies). Error bars represent means ± SD.