Table 2. Catalytic properties of recombinant fumarase D (ECs0757; ECATCC700728_0849) from E. coli O157:H7 (ATCC 700728).

Substrate	V max (U mg-1 protein)	K m (mM)	k cat/K m (M-1 s-1)
Fumarate	671 ± 28	0.9 ± 0.1	7.5 × 105
(S)-Malate	52 ± 2	0.2 ± 0.025	2.6 × 105
Mesaconate	292 ± 13	0.15 ± 0.02	2 × 106
(S)-Citramalate	104 ± 4	0.92 ± 0.10	1.1 × 105
(S,S)-(-)-Tartrate	0.13 ± 0.01	2.6 ± 0.6	50

Values are means ± standard deviations of results from at least three measurements.