. Author manuscript; available in PMC: 2016 Mar 1.

Published in final edited form as: Biol Chem. 2015 Mar;396(3):261–275. doi: 10.1515/hsz-2014-0256

Table 1.

Mirolase cleavage specificity.

	P4	P3	P2	P1		K_M[μM]	k_cat[s⁻¹]	k_cat/K_M [M⁻¹s⁻¹]
Suc	Ala	Ala	Pro	Glu	pNA		n.h.
Suc	Ala	Ala	Pro	Phe	pNA	62±19	2.27±0.16	(3.7±1.4)×10⁴
Suc	Ala	Ala	Pro	Val	pNA		n.h.
Suc	Ala	Ala	Pro	Leu	pNA	625±42	5.8±0.21	(9.3±0.9)×10³
Suc	Ala	Ala	Pro	Ile	pNA		n.h.
Suc	Ala	Ala	Pro	Asp	pNA		n.h.
Suc	Ala	Ala	Pro	Arg	pNA		n.h.
Suc	Ala	Ala	Pro	Ala	pNA	713±259	1.24±0.25	(1.7±1)×10³
MSuc	Ala	Ala	Phe	Ala	pNA		n.h.
	Suc	Phe	Pro	Phe	pNA		n.h.
	Suc	Ala	Ala	Ala	pNA		n.h.
	Suc	Ala	Ala	Val	pNA		n.h.
	Z	Gly	Gly	Leu	pNA		n.h.
	ptos	Gly	Pro	Lys	pNA		n.h.
	Suc	Val	Pro	Phe	pNA		n.h.
	Suc	Phe	Pro	Phe	pNA		n.h.
		Ala	Ala	Phe	pNA		n.h.
		Val	Leu	Arg	pNA		n.h.
		Suc	Gly	Arg	pNA		n.h.
		Z	Gly	Pro	pNA		n.h.
			Ala	Phe	pNA		n.h.
			benzo	Arg	pNA		n.h.
				Phe	pNA		n.h.
				Leu	pNA		n.h.
				Gly	pNA		n.h.

The activity assay was performed in 50 mM Tris-HCl, pH 8.0. with the appropriate substrate at final concentration 250 μM. Substrates hydrolysed by mirolase are in bold. (n.h. – no hydrolysis).