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. 2015 Dec 17;10(12):e0144845. doi: 10.1371/journal.pone.0144845

Fig 1. Silencing of PEG10 suppressed trophoblast proliferation and invasion in chorionic villous explants.

Fig 1

(A, B) Primary human chorionic villous cells (CVC) were prepared from first trimester placentas and infected with lentiviruses harboring PEG10 siRNA or non-targeting control (NC) sequence. At 48 h after infection, the expression levels of (A) PEG10 mRNA and (B) PEG10 protein were determined by real-time PCR and Western blot analysis, respectively. Data are expressed as the mean ± SD of three independent experiments. Significance versus non-transduced CVC, ***p<0.001. (C) Chorionic villi were prepared from first trimester human placentas, and subjected to syncytial denudation to remove the syncytiotrophoblast layer, followed by lentiviral transduction with PEG10 siRNA or NC. The expression of cytokeratin 7 (CK7), Ki67, HLA-G and integrin α5 in the chorionic villous explants was examined by Immunohistochemistry 72 h later. The sections were observed and photographed at 400× magnification (20 μm scales).