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. 2015 Dec 17;10(12):e0145172. doi: 10.1371/journal.pone.0145172

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© 2015 Yan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — Representative images showing the different shapes and distributions of ICMs in mouse ‘8’-shaped hatching blastocysts: compact ICMs were far away from zona opening (upper panel); scattered ICMs were around the small zona opening (middle panel); double ICMs with two obviously separated masses divided into each of the herniated blastocoels and were around the small zona opening (lower panel). ICMs were stained with anti-OCT-4 antibodies (red). All nuclei were labeled with Hoechst 33342 (blue). The scale bar is 50 μm.