Fig. 5.

Cell manipulation using of the reusable acoustic tweezers. (a) Images of 2D cell patterning with coupling method (without and with SSAW field). (b) Time-lapsed images of trapped cells (HeLa S3) in a disposable device after being trapped, removed from the LiNbO3 substrate, and cultured in the incubator for a half-hour. (c) Cell viability test using WST-1 assay. HeLa cells were treated through the disposable device with or without coupled SSAWs or incubated at 65°C for 1 hour. Cells without treatment or culture medium were used for a positive or negative control. Scale bar: 50 μm.