Skip to main content
. 2015 Dec 17;10(12):e0144937. doi: 10.1371/journal.pone.0144937

Fig 4. The expression and function of the Photorhabdus natural product rhabduscin.

Fig 4

(A) Artemis views of the RNA-seq reads of the three replicates mapped onto the Pa ATCC43949 operons responsible for rhabduscin synthesis. The isnAB genes are responsible for synthesis of the aglycon precursor shown above the left panel. The PAU_02755–7 genes encode glycosidase enzymes that add the sugar groups to produce the final rhabduscin molecule. Note PAU_02756 is unique to the P. asymbiotica (replaced by a transposase in Pl TT01) and so the final Pa ATCC43949 rhabduscin structure from Pa ATCC43949 may not be the same as that shown from Pl TT01 (above the right panel). (B) The purified aglycon precursor of rhabduscin (shown above the key) is able to completely inhibit the human alternative complement pathway. (C) Cell free supernatants from Pa ATCC43949 (PaATCC43949), Pa Kingscliff (Pa Kc) and Pl TT01 (Pl TT01) can all inhibit the human alternative complement pathway (AP). Note the classical (CP) is only partially inhibited, while LB alone also inhibits the Maltose binding lectin (MBLP) pathway to some extent.