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. 2015 Dec 17;10(12):e0145226. doi: 10.1371/journal.pone.0145226

Fig 1. PME-1 knockout reduces whole cell PP2A activity.

Fig 1

(A) Loss of PME-1 suppresses whole cell PP2A activity. PP2A phosphatase activity was analyzed with phospho-peptides as a substrate and calculated as difference between samples ± 10 nM okadaic acid. N = 4. *: P<0.05 vs. WT. (B) Loss of PME-1 suppresses PP2A specific activity. PP2A specific phosphatase activity was analyzed with immunoprecipitation based PP2A activity assay. N = 4. *: P<0.05 vs. WT. (C-D) Loss of PME-1 enhances PP2Ac methylation. Cell lysates were treated with or without NaOH. The ratio of demethylated PP2Ac was determined by immunoblotting for demethylated PP2Ac (deMet PP2Ac). Representative picture (C) and quantitative data (D) from 3 independent experiments are shown. *: P<0.05 vs. WT.