Table 1.

Characterization of 21 microsatellite loci in Noccaea caerulescens.

Locus	Primer sequences (5′–3′)	Repeat motif	Ta (°C)	Ct (µM)	Multiplex	Fluorescent dye	Post-PCR dilution	Position A.t.a	Position N.c.a	Chromosomal blocksb	Allele size range (bp)	GenBank accession no.	Publication
Ncpm09	F: TAGACGCTGCGTTTTGAAGA	CT18	58	0.4	NcM1	6-FAM	1/150	1	2	B	96–130	KR065729
	R: CCTCTGTTGAGTGAATGGTTCTC
Ncpm13	F: CCAAAACTATGCCGATCTCA	AG16	58	0.2	NcM1	VIC	1/150	NA	NA	NA	158–166	KR065730
	R: CCACGAGCGAATCTTCTTGT
Ncpm21	F: GTCACCACTTGCTACGGGAT	CTT10	58	0.2	NcM1	VIC	1/150	3	7	F	240–277	KR065731
	R: CATTGGATAGCACAGAGGCA
Ncpm23	F: TTTCATGTCTCGGATCCTCC	TTC11	58	0.4	NcM1	6-FAM	1/150	NA	NA	NA	197–263	KR065732
	R: GCAGAGCGCAATCTAAGGAC
Ncpm31	F: GATTATCGAGCTTACTAAAAGCAGC	CTT12	58	0.2	NcM1	NED	1/150	5	4	W	58–101	KR065733
	R: GTGTTGAAGCGCAATGAAGA
Tc-up1	F: TGCTCTGTTTCTCTCCACATTC	(CA)n(CT)n	50	0.2d	NcM1	NED	1/160	NA	NA	NA	132–170	AJ746212	Basic and Besnard, 2006
	R: TTCCTTGCTTCTTCTCTTCCA
Ncpm07	F: TGGAATGGTTCTGTGGACAA	TCA18	58	0.4	NcM2	6-FAM	1/150	NA	NA	NA	109–139	KR065734
	R: TTCTGGAATTGGCTGCTTCT
Ncpm14	F: GACCACATCTCGTCTTGCCT	CTT15	58	0.4	NcM2	PET	1/150	NA	NA	NA	108–123	KR065735
	R: GACCCTAACTACAGGCTGTGAAA
Ncpm19	F: CCAACAATGGATTGGGAGAG	GTGTA(TG)14	58	0.4	NcM2	VIC	1/150	NA	NA	NA	100–124	KR065736
	R: TCCCCATCACTCCAGCTAAG
Ncpm29	F: CTCCCTCTTCTCCTACCTACACATA	AC17	58	0.4	NcM2	NED	1/150	NA	NA	NA	78–94	KR065737
	R: GGTTGAAAGTAGGAGTGAGTCAAGA
Tc-up4	F: GTTTTGTCCGCTTTGCTTCC	CT13	50	0.2d	NcM2	VIC	1/140	NA	NA	NA	253–262	AJ746216.1	Basic and Besnard, 2006
	R: GCCATAGACTTTCTCATTGATTC
9C7/Thlc3	F: GTCACGAGTTTCACCATT	AG13	58	0.4	NcM2	VIC	1/150	NA	NA	NA	152–191		Jiménez-Ambriz et al., 2007
	R: ATCTTCCACAATTGTGCC
Tc-up2	F: TGAGAAGAGGAGACACAGGAAC	(AG)5(AG)5(GA)6	58	0.2	NcM2	PET	1/150	NA	NA	NA	232–244	AJ746213.1	Basic and Besnard, 2006
	R: CACTTACCAAATCGAAAACTGCTCC
Nc02	F: GGAGCTGTGGTTTCTGAAGG	AGG8	68/47c	0.06	NcM3	VIC	None	4	3	O	170–191	KR065738
	R: AGCATCGTATTCCGATCCAG			0.28
Nc03	F: TGAGCTTCTTCAGTCCCGAT	AC12	68/47c	0.06	NcM3	NED	None	5	4	S	188–194	KR065739
	R: TATGAGCTCGTCGCTCACAG			0.28
Nc04	F: ACGGTCGCATACCAAAAAGT	AG11	68/47c	0.06	NcM3	VIC	None	2	5	J	123–143	KR065740
	R: AGGATGCACTCCTTGAGACC			0.28
Nc06b	F: GCGTCTTCTCTCCATCCTCA	AG13	68/47c	0.07	NcM3	PET	None	4	6	U	89–153	KR065741
	R: GGATTTCCAATTCAATCTTCCC			0.37
Nc07b	F: CCAGTTTCCAACGGCATAGT	AC10	68/47c	0.06	NcM3	NED	None	3	7	F	105–112	KR065742
	R: TTGGTTTGGTTTCTTCTTGTGA			0.28
Nc19	F: CGGATTGTTGTGAATCCCAT	AGG11	68/47c	0.07	NcM3	PET	None	2	5	J	199–235	KR065743
	R: ACCTCTTCTTTCGCCCTTGT			0.28
Nc20	F: ACACAACTCCAAAGGCTTCA	AG11	68/47c	0.15	NcM3	6-FAM	None	4	3	O	205–234	KR065744
	R: TTTTGTTCTAACCGTTACTCTTT			0.75
Nc22	F: TTGCTTTCACATGTCTTGACG	AG10	68/47c	0.11	NcM3	6-FAM	None	2	5	K	111–121	KR065745
	R: GAACAGAACAAGAAGACATGAATGA			0.56

Note: Ct = final concentration of primers; NA = not available; T_a = annealing temperature.

^aRelative chromosomal position in Arabidopsis thaliana and Noccaea caerulescens, respectively (Schranz et al., 2006).

^bGenome blocks in the “ancestral karyotype” blocks (Schranz et al., 2006).

^cFollowing Godé et al. (2012), touchdown PCR was performed. During the first 10 cycles, annealing temperature was decreased from 68°C to 50°C in increments of 2°C. This was followed by 27 cycles with an annealing temperature at 47°C.

^dStandalone PCR.