Appendix 1.
Voucher and location information for Noccaea caerulescens populations used in the development and testing of microsatellites.
| Population | Type of population | Collection date | Locality | Geographic coordinates | Sample names and storage locationa | Voucher no. | Use |
| Angleur | Metallicolous | 2009 | Angleur | 50°36′44.61″N, 5°36′38.74″E | B02.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Anjeau | Nonmetallicolous | October 2007 | Saint-Laurent-le-Minier | 43°55′3.33″N, 3°37′52.52″E | AN_Z_X_2007c | Biological validation of primer pairs (ISEM) | |
| Auxelles-Haut | Nonmetallicolous | 2009 | Auxelles-Haut | 47°44′21.52″N, 06°46′35.84″E | F03.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Auxy | Nonmetallicolous | 2009 | Auxy | 46°57′44.13″N, 04°23′47.05″E | F05.01b,d | 503854 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Avinières | Metallicolous | March 2013 | Saint-Laurent-le-Minier | 43°55′55.67″N, 3°39′46.19″E | 12_AV_ID_Xc | Natural populations screening | |
| Baraquette | Nonmetallicolous | October 2007 | Saint-Laurent-le-Minier | 43°55′6.73″N, 3°36′54.82″E | BQ_Z_X_2007c | Library construction | |
| Breinigerberg | Metallicolous | 2009 | Breining | 50°44′12.73″N, 06°14′30.92″E | G01.01b,d | 501266, 501267 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Col du Lautaret | Nonmetallicolous | 2007 | Lautaret | 45°02′07″N, 06°24′20″E | F01.01b,d | 501429 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Coulet | Nonmetallicolous | September 2013 | Saint-Maurice-Navacelles | 43°49′44.59″N, 3°33′41.64″E | 12_CO_ID_Xc | Natural populations screening | |
| Coulet | Nonmetallicolous | October 2007 | Saint-Maurice-Navacelles | 43°49′44.59″N, 3°33′41.64″E | CO_Z_X_2007c | Biological validation of primer pairs (ISEM) | |
| Fellering | Serpentine | 2009 | Bergenbach | 47°54′22.90″N, 06°57′25.50″E | F04.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Goebelsmühle | Nonmetallicolous | 2009 | Goebelsmühle | 49°55′22.07″N, 06°3′44.08″E | L02.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Husavik | Nonmetallicolous | 2006 | Husavik | 66°01′38.89″N, 17°17′21.71″W | I01.01b,d | 911812 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Lichtenau | Metallicolous | 2006 | Blankenrode | 51°32′0.34″N, 08°54′17.02″E | G05.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Lintich | Metallicolous | 2010 | Bakomi | 48°26′05.48″N, 18°55′09.43″E | SK01.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Mostviertiel | Nonmetallicolous | 2006 | Tormäuer | 47°51′08.70″N, 15°17′13.20″E | A01.01b,d | 406733 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Moyen Age | Metallicolous | October 2007 | Saint-Laurent-le-Minier | 43°55′52.16″N, 3°38′26.09″E | MG_Z_X_2007c | Biological validation of primer pairs (ISEM) | |
| Oslo | Nonmetallicolous | 2009 | Hovedoya | 59°53′39″N, 10°43′44″E | N01.01b,d | 501275–501278 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Papeterie | Metallicolous | 2006 | Ganges | 43°56′10.98″N, 03°40′19.88″E | F16.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Prayon | Metallicolous | 2009 | Prayon | 50°35′3.31″N, 5°40′23.69″E | B01.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Ramponenche | Metallicolous | 2010 | Florac | 44°20′18.25″N, 03°40′05.45″E | F02.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Saint Baudille | Nonmetallicolous | October 2007 | Montpeyroux | 43°44′40.73″N, 3°29′9.96″E | BD_Z_X_2007c | Biological validation of primer pairs (ISEM) | |
| Saint Bresson | Metallicolous | October 2007 | Pommiers | 43°56′35.89″N, 3°37′57.02″E | SB_Z_X_2007c | Biological validation of primer pairs (ISEM) | |
| Saint Bresson | Metallicolous | September 2013 | Pommiers | 43°56′35.89″N, 3°37′57.02″E | 12_SB_ID_Xc | Natural populations screening | |
| Saint Hippolyte | Metallicolous | April 2014 | Saint-Hippolyte-du-Fort | 43°58′17.07″N, 3°49′59.98″E | Zi_Qj_Hi_2014_Xc | Natural populations screening | |
| Saint Jost | Metallicolous | 2009 | Virneburg | 50°21′08.95″N, 07°06′33.65″E | G02.01b,d | 501261, 501262 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Silberberg | Metallicolous | 2009 | Silberberg | 52°12′38.72″N, 07°56′46.13″E | G03.01b,d | 504547 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Somerset | Metallicolous | 2010 | Priddy | 51°15′39.06″N, 02°39′02.12″W | UK1.06b,d | 501343 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Son | Nonmetallicolous | 2000 | Esterri d’Aneu | 42°35′51.50″N, 01°04′23.82″E | SP01.02b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Špania Dolina | Nonmetallicolous | 2010 | Bakomi | 48°48′29.87″N, 19°08′14.65″E | SK02.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Stauffenbergallee | Nonmetallicolous | 2009 | Dresden | 51°06′04.48″N, 13°47′17.61″E | G04.01b,d | 280040 | Biological validation of primer pairs (Evo-Eco-Paleo) |
| Štiavnické | Nonmetallicolous | 2010 | Bakomi | 48°26′0.48″N, 18°51′29.86″E | SK03.01b | Biological validation of primer pairs (Evo-Eco-Paleo) | |
| Uppsala | Nonmetallicolous | 2010 | Uppsala | 59°50′40.68″N, 17°44′54.18″E | S01.01b,d | 501430 | Biological validation of primer pairs (Evo-Eco-Paleo) |
a
Letters in sample names are defined as: X = number of the plant on which a leaf was collected; Zi = number of the area in the population; Qj = number of the quadrat in the area; Z = plants on which seeds were collected. Several specimens were collected in each population.
b
Vouchers of leaves were deposited at Laboratoire Évolution Écologie et Paléontologie (Evo-Eco-Paleo), Villeneuve d’Ascq, France.
c
Vouchers of leaves were deposited at Institut des Sciences de l’Évolution (ISEM), Montpellier, France.
d
Herbarium specimens of the corresponding population were collected and deposited by M. Koch at the Centre for Organismal Studies (COS), Biodiversity and Plant Systematics, Universität Heidelberg, Heidelberg, Germany. They are available from the BrassiBase database (Kiefer et al., 2014).