Table 1.
Characteristics of 16 microsatellite loci developed in Rumex bucephalophorus subsp. canariensis.
| Locus | Primer sequences (5′–3′)a | Repeat motif | Ta (°C) | Allele size range (bp) | GenBank accession no. |
| 441 | Fm: GGAAACAGCTATGACCATCACTCGACATCACTCCAATC | (AG)9 | TD 49.5 | 181–193 | KT351066 |
| R: GTTTAGCGAATGATGAAGAATGAC | |||||
| 510 | Fm: GGAAACAGCTATGACCATAATGAAGGAAATGACGACTG | (AC)9 | 52 | 127–181 | KT351068 |
| R: GTTTAGGTCAGTTCGATTGTGTTG | |||||
| 679 | Fm: GGAAACAGCTATGACCATACTCACACTCACGCATGTC | (ACT)7 | 49 | 148–202 | KT351064 |
| R: GTTTGTTGATCCTGTCGAATGTTG | |||||
| 1087 | F: GTTTACCGTCCGATAACAATCTAG | (ACTC)6 | 52 | 196–204 | KT351056 |
| Rm: GGAAACAGCTATGACCATCTATAAGTATCGTGCGTGTTG | |||||
| 1322 | F: GTTTATTTACATGGTGCGAGAAAG | (AG)10 | 55 | 301–403 | KT351065 |
| Rm: GGAAACAGCTATGACCATCACAATGCATGACGTTTAAC | |||||
| 1327 | F: GTTTCCACGAGTTGTAACACAC | (AC)12 | 52 | 88–153 | KT351067 |
| Rm: GGAAACAGCTATGACCATCTATAAGTATCGTGCGTGTTG | |||||
| 1648 | Fc: CAGTCGGGCGTCATCAAATTCGAGCAACAGTAAG | (AAAC)7 | 52 | 176–204 | KT351061 |
| R: GTTTGGCACCTTAGACACATTCAC | |||||
| 1806 | Fm: GGAAACAGCTATGACCATCCCACACTCTTCTGTCATG | (AAC)15 | 55 | 264–294 | KT351053 |
| R: GTTTAGAGGCTGCATACAACAAG | |||||
| 1959 | F: GTTTGAGTCACAGCCCAAGATTAG | (AAC)7 | 55 | 304–340 | KT351055 |
| Rc: CAGTCGGGCGTCATCAGGTGGTCGAGAAAGTAGTG | |||||
| 1965 | F: GTTTGGCGAGTTATTCCTCTTATG | (AAAC)7 | 52 | 160–180 | KT351062 |
| Rm: GGAAACAGCTATGACCATAATCCAAGACTTCTGCAATC | |||||
| 2031 | Fm: GGAAACAGCTATGACCATCTATAAGTATCGTGCGTGTTG | (ACTC)15 | 52 | 124–128 | KT351057 |
| R: GTTTAACTCTATATGTTTGCCTCCTC | |||||
| BHZNV | Fm: GGAAACAGCTATGACCATTCCCTCCACCTAACAAAG | (ACAT)8 | TD 49.5 | 124–128 | KT351060 |
| R: GTTTATCACACGCCGTTAAATATC | |||||
| CUN6O | Fm: GGAAACAGCTATGACCATCCACCCTACAAATCATGAG | (AAC)9 | 55 | 165–201 | KT351054 |
| R: GTTTACCTTCATAATCTGCCAAAG | |||||
| D8UYR | Fm: GGAAACAGCTATGACCATTTAATCCCTTGCATTCTTC | (AAAG)7 | 52 | 160–196 | KT351058 |
| R: GTTTACCCAAGAGAGCTGGTTAG | |||||
| D90ZA | F: GTTTCTGATTACACCTTCGGAAC | (AAAG)8 | 49 | 119–263 | KT351063 |
| Rc: CAGTCGGGCGTCATCATTTGTCTAAGCCGTTCAAG | |||||
| EJ5P7 | F: GTTTATGAGGTTCCTACGTTGTTG | (ACAT)8 | TD 49.5 | 188–216 | KT351059 |
| Rm: GGAAACAGCTATGACCATACGTACCGATGAAGAAGTTG |
Note: Ta = annealing temperature; TD = touchdown PCR procedure.
a
m = modified primer with underlined M13R motif; c = modified primer with underlined CAG motif. PIG-tail primers are set boldface.