Fig 5. Fenofibrate demonstrated iGSM activity in cell-free assay.

To confirm GSM activity, fenofibrate was added to crude membrane extracts during incubation and Aβ peptides recovered by immunoprecipitation and analyzed by western blotting using 82E1 antibody. In (A), addition of fenofibrate showed elevation of Aβ42 species. Shorter peptides such as Aβ38 and Aβ39 cannot be resolved due to overlap of APP C-terminal fragments in this gel system. Quantification of results from (A) is shown in (B) from the average of four experiments ± S.E. (* p < 0.05 two-tailed paired Student’s t test).