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. 2015 Dec 17;10(12):e0144758. doi: 10.1371/journal.pone.0144758

Fig 7. FAD-linked PS1 mutation showed reduced γ-secretase activity and also shifted position of ε-cleavage.

Fig 7

CHO cells stably expressing wild type PS1 or A79V or L166P mutations were analyzed form AICD production. (A) Both PA1 mutations reduced γ-secretase activity as determined by the rate of AICD generated from crude cell membranes up to 60 minutes (n = 3; averages ± S.E). (B) By immunoprecipitation and western blotting, levels of AICD50 and AICD 51 were unchanged after addition of flurbiprofen or fenofibrate. (C) Quantification of results from (B) showed no significant changes in AICD50/AICD51 ratios after drug treatment (n = 3, “NS”: p > 0.05 one-way ANOVA; averages ± S.E.).