Fig 7. Flow cytometric analysis for apoptosis of fibroblasts.

Representative dot plots of the flow cytometric analysis of the Annexin V–FITC/propidium iodide (PI) following induction of apoptosis with H₂O₂. Fibroblasts derived from SM-exposed patients and controls were assessed for spontaneous apoptosis and apoptosis following treatment with 500, 700 and 900 μM of H₂O₂. Spontaneous apoptosis was not significantly different in SM-exposed patients and controls. However, a higher dose-dependent sensitivity to H₂O₂ is observed in patients as compared with controls. Q₁: necrotic cells, Q₂: late apoptotic cells, Q₃: viable cells, and Q₄: early apoptotic cells.