Fig 6. bdg1 endosperm exhibits higher permeability to toluidine blue.

(A) Comparison of toluidine blue staining in embryos dissected from WT and bdg1 seeds. Whole seeds of WT and bdg1 were incubated on MS, ABA and PAC for different times prior or after TR as indicated. Thereafter, seed material was transferred to a toluidine blue solution for 6 hours. The solution maintained PAC or ABA if these compounds were previously present in the germination medium. For illustration, a picture of a seed with or without TR is shown. Pictures show embryos dissected out of seed coats after 6h incubation in toluidine blue solution. Histograms show blue intensity quantification obtained from pictures (Materials and Methods). (*** = P-values < 0.001).