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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Oncogene. 2015 Jun 1;35(9):1153–1162. doi: 10.1038/onc.2015.170

Figure 6. PSGR can regulate stromal androgen receptor (AR) in vitro in the presence of inflammatory cells.

Figure 6

A. Reverse transcription-PCR of AR signaling-related gene products shows decreased AR and target gene mRNAs (NKX3.1, PSA) following PSGR overexpression. N = 2. B. Co-culture experiments using the stromal cell line WPMY-1 with indicated additional cell lines show increased WPMY-1 PSGR mRNA expression in the presence of both PSGR-overexpressing LnCaP cells and the Raw264.7 macrophage cell line (p < 0.0002, t-test). Bar graph represents mean ± standard error of n=3 experiments, each done in triplicate. C. Co-culture with PSGR overexpressing LnCaP cells induced WPMY-1 cell AR mRNA expression only in the presence of Raw264.7 macrophages (p<0.06). Bar graph represents mean ± standard error of n=3 different experiments, each in triplicate.