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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Cell Biochem. 2016 Jan;117(1):144–151. doi: 10.1002/jcb.25259

Figure 3.

Figure 3

SAHA treatment enhances the ALAD-proteasome interaction, associated with acetylation of ubiquitinated α2 subunits. A) SAHA treatment enhances ALAD protein levels in a dose-dependent manner. Empty vector or myc-tagged full length ALAD-transfected PC-3 cells were treated with 5 or 10 µM SAHA for 24 h followed by Western blot analysis. B) SAHA enhances the ALAD-proteasome interaction and promotes acetylation of ubiquitinated α2. Myc-tagged full length ALAD-transfected cells were treated with 10 µM SAHA for 24 h followed by immunoprecipitation (500 µg) with anti-myc and analysis by Western blot. Matched IgG immunoprecipitation was performed as a control. Input was loaded at 5% of total protein used for immunoprecipitation. V = Vector-transfected; A = ALAD-transfected; + = SAHA-treatment; − = DMSO treatment; P = Pulldown; S = Supernatant; I = Input.