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. Author manuscript; available in PMC: 2015 Dec 17.
Published in final edited form as: Bioorg Med Chem Lett. 2015 Sep 1;25(20):4464–4471. doi: 10.1016/j.bmcl.2015.09.001

Figure 3.

Figure 3

Uncaging of intracellular caged auxin in planta. 5-days-old DR5::GUS seedling was incubated in caged auxin solution for 30 min and then the seedlings were washed with fresh medium. After loading of caged auxins, the seedlings were placed on agar medium plate. The intracellular caged auxins were uncaged by light irradiation and then the seedling was incubated for 5 h in the dark. The auxin-induced GUS enzyme activity was visualized by histochemical blue staining. The values in parenthesis represent the concentration (μM) of caged auxin solution used for loading.