Table 1.
Primers and annealing temperatures used for PCR assays designed to investigate the prevalence of ctenophore-associated circular viruses (CtaCVs).
| Virus name | Primer sequences (5′–3′) | Annealing temperature (°C) | Expected product size (bp) |
|---|---|---|---|
| CtaCV-1 | F-CCA CCA GAC TGG GAC GTA GT R-ACA GCC GTC AAA CCA TTT TC |
58 | 555 |
| CtaCV-2 | F-AGA ACA GGG AAC TCC CCA CT R-CGC AAC GTT CAC AAA GAA GA |
54 | 592 |
| CtaCV-3 | F-ATG CCT GGT TAC CAA TTT CG R-TCA AGC CAT CGA GTT TTT CC |
58 | 521 |
| CtaCV-4 | F-GCA AGA CAC AGC TGG AAA CA R-CTC CAC GAA GCT TTT TAC CG |
50 | 593 |