FIGURE 5.

Coverage-GC plots for the synthetic AB metagenome (plot symbols are scaled by contig length). (a) Coverage levels show decrease at extreme GC values; note logarithmic scale in vertical axis. Crosshairs mark contigs with SSU rRNA genes, which tend to have higher coverage and more moderate GC values on average than other contigs. (b) Genome bins produced by MetaBAT as colored overlays (colors arbitrary), showing that not all contigs were assigned to a bin, including some high-coverage contigs; unbinned contigs shown in gray. The genome of Nanoarchaeum equitans assembled into a single contig (arrow) and was therefore not assigned to a bin by MetaBAT.