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. 2015 Oct 7;309(12):C835–C846. doi: 10.1152/ajpcell.00107.2015

Fig. 9.

Fig. 9.

EPEC infection reduces DRA association with lipid rafts. A: Caco-2 monolayers were infected with EPEC for 60 min. Total membrane pellet was obtained by ultracentrifugation. Lipid rafts were separated by solubilization in detergent buffer at 4°C. Lipid raft [detergent-insoluble (DI)] fractions were resolved on a 7.5% SDS-polyacrylamide gel and immunoblotted with anti-DRA and anti-actin antibodies. Results are shown as ratio of DRA to β-actin. Values are means ± SE of results from 4 different experiments. **P < 0.001 vs. control. B: Caco-2 monolayers were infected with EPEC in the presence or absence of dynasore for 60 min. Total membrane pellet was obtained from control and EPEC-infected cell lysates by ultracentrifugation. Lipid rafts were separated by solubilization in a Triton X-100 detergent buffer at 4°C. Lipid raft (DI) fractions were resolved on a 7.5% SDS-polyacrylamide gel and immunoblotted with anti-DRA and anti-actin antibodies. Results are shown as ratio of DRA to β-actin. Values are means ± SE of results from 4 different experiments. **P < 0.001 vs. control.