Fig. 1.

Multilevel fractionation approach used to enable “deep” proteomic profiling of mouse mpkCCD cells. Differential centrifugation was used to prepare 5 fractions from homogenized renal mpkCCD cells: 1,000-, 4,000-, 17,000-, and 200,000-g pellets and a 200,000-g supernatant. Each fraction was further separated by SDS-PAGE, and each lane was divided into 43 slices, each of which was subjected to in-gel trypsinization and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. dDAVP, 1-desamino-8-d-arginine-vasopressin; Sup, supernatant; m/z, mass-to-charge ratio.