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In mpkCCD cells, ubiquitin peptides are found over a broad range of molecular weights, consistent with widespread protein ubiquitylation. A virtual Western blot is shown for all fractions, with vehicle (“ctrl”) vs. vasopressin (“dDAVP”) treatment. Ubb and Ubc code for identical ubiquitin polypeptides. Logarithmic mapping of values to gray-scale levels was used.
