Fig. 4.

A publically available web page was created to provide users with a comprehensive listing of proteins expressed in mpkCCD cells with virtual Western blots for each protein. Top: general appearance of the web page accessible at https://helixweb.nih.gov/ESBL/Database/mpkFractions/. Green box: relative abundances in each fraction in heat-map format; blue box: changes with dDAVP in each fraction from stable isotopic labeling by amino acids in cell culture (SILAC) quantification in heat-map format (increase, red; decrease, yellow; no change, white). To display a virtual Western blot, mapping mass spectrometry-identified peptides back to the original SDS-polyacylamide gel position, the user can mouse-over or click on a particular GI number (illustrated for A-Raf at bottom left). Virtual Western blot is shown for all fractions, with vehicle (“ctrl”) vs. vasopressin (“dDAVP”) treatment. Virtual Western blot for A-Raf can be compared with an actual Western blot (bottom right).