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Mobilities of proteins on SDS-PAGE are in part dependent on protein isoelectric point (pI). pI, calculated for the whole protein, is plotted for 3 groups of proteins segregated on the basis of ρ range for each of 5 differential-centrifugation fractions. *P < 0.025, low-ρ or high-ρ group vs. intermediate group (ρ = 0.65–1.45), by ANOVA with Bonferroni's contrasts. Bottom: standard errors, total number of proteins, and P values.
