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. 2015 Oct 23;290(51):30212–30223. doi: 10.1074/jbc.M115.696799

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — HPLC analysis of NOV2-catalyzed reaction products and the influence of O₂ depletion and supplementation on NOV2 enzymatic activity. A, HPLC trace of NOV2-catalyzed reaction products generated from cleavage of resveratrol. The peak assignments were made based on their elution times, in comparison with authentic standards, and their characteristic absorbance spectra are shown to the right of the HPLC. Absorbance maxima for resveratrol, 4-HBA, and 3,5-DHBA were 304, 271, and 267 nm, respectively. mAU, milliabsorbance unit. B, NOV2 activity assays were performed either without argon pretreatment to displace O₂ (control) or after a 10- or 15-min argon purge (white bars) and compared with NOV2 samples undergoing the same treatments but subjected to 3 min of O₂ gas supplementation as a final step (gray bars). Error bars represent S.D.s calculated from measurements performed in triplicate.