FIGURE 3.

Sequence alignment of OGA homologues from T. terrenum, O. granulosus, C. perfringens, and H. sapiens (h). Sequence numbering and secondary structure annotation are in accordance to the T. terrenum OGA structure. α-Helices are indicated by barrels, and β-sheets are indicated by arrows (TIM barrel, green; helical bundle domain, orange). The disordered region and the histone acetyltransferase domain of hOGA are indicated with dashed boxes colored pale yellow and dark red, respectively. The extended α10-α11 loop of CpOGA is marked with a cyan dashed box. The blue triangle marks the catalytic double aspartate motif, the red triangles indicate the residues that tether the O-GlcNAc moiety of the substrate, and the dark red triangles indicate the residues that play a role in recognition/positioning of the peptide in TtOGA.