FIGURE 4.

TtOGT and TtOGA are expressed throughout the exponential phase of growth and are intercellular proteins. A, the growth of T. terrenum was followed in NYZ medium supplemented with 0.2% glucose for 92 h. The optical density of the cultures was measured at 600 nm. The curve shown is an average of three independent biological replicates. The error bars represent S.D. from the mean. B, the samples of the cell pellet (P) and filtered medium supernatant (Sn) taken at 24, 48, and 72 h postinoculation were analyzed for the presence of TtOGT, TtOGA, and housekeeping σ factor RpoD. The supernatant fraction was concentrated 400-fold prior to the analysis. C, loading control of the anti-TtOGA and anti-TtOGA Western blot. Identical samples of the cell pellet lysate and precipitated medium supernatant fractions were resolved by 10% SDS-PAGE and stained with InstantBlue. Images of all lanes were acquired from different parts of one gel.