FIGURE 6.

Cell-permeable OGT inhibitor precursor Ac₄-5S-GlcNAc is bacteriostatic to T. terrenum. A, T. terrenum cells were treated with increasing concentrations of Ac₄-5S-GlcNAc from 15 to 1,000 μm in 100 μl of DMSO, and the culture growth was followed for 72 h by measuring optical density at 600 nm. The resulting growth curves were compared with growth of the untreated culture as well as that treated with a vehicle control (100 μl of DMSO). B, the effect of Ac₄-5S-GlcNAc on the growth of T. terrenum is dose-dependent. The half-maximal effective concentration of Ac₄-5S-GlcNAc (509 μm) was calculated from the A₆₀₀ values at the 62-h time point. The data are representative of three independent biological replicates. The error bars represent S.D. from the mean. C, Ac₄-5S-GlcNAc treatment has no effect on growth of B. subtilis. The growth of B. subtilis without treatment (black circles), with treatment with vehicle control (100 μl of DMSO) (open squares), or with treatment with 1 mm Ac₄-5S-GlcNAc in 100 μl of DMSO (open triangles) was measured every 45 min over a 500-min time course by measuring the optical density at 600 nm.