TABLE 1.

Sequences of oligonucleotides used as primer for cloning of human SIRT1 promoter and real time QPCR

Primer	Sequence
For SIRT1 promoter
PF-1 (−1200)	5′-CTCGAGAACCCATACTAGGCTTAAGGG-3′
PF-2 (−691)	5′-CTCGAGATCGGCTGATCTCCAAAC-3′
PF-3 (−457)	5′-CTCGAGAGAACGACTATCCAAC-3′
PF-4 (−47)	5′-CTCGAGGTTTAAATCTCCCGCAG-3′
PR-1 (+53)	5′-AAGCTTCTTCCAACTGCCTCTCTG-3′
For QPCR
SIRT1	Forward, 5′-TGGCAAAGGAGCAGATTAGTAGG-3′
	Reverse, 5′-CTGCCACAAGAACTAGAGGATAAGA-3′
CHOP	Forward, 5′-ATGGCAGCTGAGTCATTGCCTTTC-3′
	Reverse, 5′-AGAAGCAGGGTCAAGAGTGGTGAA-3′
ERdj4	Forward, 5′-AGTAGACAAAGGCATCATTTCCAA-3′
	Reverse, 5′-CTGTATGCTGATTGGTAGAGTCAA-3′
GRP78	Forward, 5′-ACCAATTATCAGCAAACTCTATGGAA-3′
	Reverse, 5′-CATCTTTTTCTGCTGTATCCTCTTCA-3′
XBP1	Forward, 5′-CCGCAGCAGGTGCAGG-3′
	Reverse, 5′-GAGTCAATACCGCCAGAATCC-3′
ATF4	Forward, 5′-AGTGGCATCTGTATGAGCCCA-3′
	Reverse, 5′-GCTCCTATTTGGAGAGCCCCT-3′
18S rRNA	Forward, 5′-CGGCTACCACATCCAAGGAA-3′
	Reverse, 5′-GCTGGAATTACCGCGGCT-3′
mSIRT1	Forward, 5′-TTGCAAAGGAGCAGATTAGTAAGC-3′
	Reverse, 5′-TGCCACAGGAACTAGAGGACAA-3′
mIL-6	Forward, 5′-TAGTCCTTCCTACCCCAATTTCC-3′
	Reverse, 5′-TTGGTCCTTAGCCACTCCTTC-3′
mCTGF	Forward, 5′-GGGCCTCTTCTGCGATTTC-3′
	Reverse, 5′-ATCCAGGCAAGTGCATTGGTA-3′
m18S rRNA	Forward, 5′-CCATCCAATCGGTAGTAGCG-3′
	Reverse, 5′-GTAACCCGTTGAACCCCATT-3′