FIGURE 7.
Quantitative kinetics displaying the processing of the spliced peptide gp100210–218/220–222 by 20S proteasomes and ERAP1. A, proteasome-mediated generation kinetics of the peptide gp100210–218/220–222 from the wt and mut gp100201–230 substrate digestions carried out by 20S s- and i-proteasomes. Peptide amount values are the means, and bars are the S.D. of three independent in vitro experiments. B, ERAP1 trimming kinetics of the substrate wtgp100210–218/220–222. No products shorter than the HLA-B*07:02-restricted epitope wtgp100213–218/220–222 were detected in the assay. Peptide amount values are the means, and bars are the S.D. of three independent in vitro experiments. C, binding to the HLA-B07:02 molecule by the epitope wtgp100213–218/220–222 (IC50 = 28 nm).