FIGURE 1.

BAG2 forms a complex with PINK1. A, BAG2 interacts with PINK1 upon expression of PINK1 and BAG2 in HEK293T cells by co-immunoprecipitation. The plasmids PINK1-V5 and MYC-BAG2 were co-transfected into HEK293T cells. For IP of MYC-BAG2 (left panel), transfection of an empty vector and PINK1-V5 was as one of the controls. For reverse IP of PINK1-V5 (right panel), transfection of an empty vector and MYC-BAG2 was as one of the control. IB, immunoblot. B, mitochondrial (mito) and cytosolic (cyto) fractions from HEK293T cells transfected with PINK1-FLAG and MYC-BAG2 were subjected to IP using MYC antibody. The precipitated proteins were separated by SDS-PAGE for Western blots. C, the total cell lysate from HEK293T was used for IP of endogenous PINK1. The endogenous BAG2 was detected from IP of PINK1. Asterisks, full-length PINK1; #, processed PINK1; > and <, the heavy chain of IgG.