TABLE 1.

Apparent affinities obtained from substrate-induced ³H-substrate uptake by SLC1A2-WT and Ser-364 mutants

K_m values from uptake experiments in HEK cells expressing SLC1A2-WT and the indicated Ser-364 mutants are shown below. K_m values were calculated from data obtained from three independent experiments and are represented as means ± S.D. (*, p < 0.05, mutant versus WT).

SLC1A2	l-Glutamate	l-Aspartate	d-Aspartate
	μm	μm	μm
WT	158.1 ± 28.7	48.1 ± 26.5	91.1 ± 40.2
S364T	161.8 ± 12.1	53.6 ± 15.3	115.1 ± 51.28
S364A	900.7 ± 84.5*	882.6 ± 642.2*	1151.2 ± 398.0*
S364C	1080.0 ± 52.0*	188.8 ± 66.1*	350.1 ± 91.6*