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. 2015 Oct 22;290(51):30530–30539. doi: 10.1074/jbc.M115.682583

FIGURE 5.

FIGURE 5.

Orai1 and TRPC1 are involved in GSIS in INS-1E cells. INS-1E cells were co-transfected with plasmids coding for the hGH and one of the following plasmids: control pmaxGFP (GFP), TRPC1-F562A, or Orai1-E106D. Transfected cells were incubated for 30 min in KRBH supplemented with 1.6, 16.7, and 16.7 mm glucose + 100 μm ACh or 16.7 mm glucose + 1 μm Tg. hGH secretion (supernatant) was normalized by the hGH content (% content) and expressed as means ± S.E. of six independent experiments. ***, p < 0.001 versus basal insulin secretion at 1.6 mm glucose; #, p < 0.05; ##, p < 0.01; ###, p < 0.001 versus insulin secretion at 16.7 mm glucose; $$$, p < 0.001 versus insulin secretion at 16.7 mm glucose + ACh; ¤¤¤, p < 0.001 versus insulin secretion at 16.7 mm glucose + Tg.