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. 2015 Oct 26;290(51):30607–30615. doi: 10.1074/jbc.M115.681627

FIGURE 2.

FIGURE 2.

Overexpression of FOXP1 in the liver of C57BL/6J mice inhibits hepatic gluconeogenesis. Male C57BL/6J mice were injected with Ad-GFP of Ad-FOXP1 as described under “Experimental Procedures.” 5–7 days after infection, mice were sacrificed and plasma and liver tissues were collected for further analysis. A, real-time PCR analysis of mRNA levels of FOXP1, PEPCK, and G6PC in the liver of Ad-GFP or Ad-FOXP1-injected C57BL/6J mice (n = 7 each group). B, Western blotting analysis of protein levels of FOXP1, PEPCK, and α-Tubulin in the liver of Ad-GFP or Ad-FOXP1-injected C57BL/6J mice (n = 7 each group). C and D, blood glucose levels of 6-h fasted (C) and 16-h fasted (D) C57BL/6J mice on day 5 after injection with the indicated adenovirus (n = 7 each group). E, pyruvate tolerance test (n = 7 each group). F, glucose tolerance test (n = 7 each group). *, p < 0.05; **, p < 0.01, mice injected with Ad-GFP versus mice injected with Ad-FOXP1.