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. 2015 Oct 26;290(51):30607–30615. doi: 10.1074/jbc.M115.681627

TABLE 1.

Primers used in PCR

F, forward; R, reverse.

Forward Reverse
Real-time PCR primers
    PGC-1α F, 5′-CATTTGATGCACTGACAGATGGA-3′ R, 5′-GTCAGGCATGGAGGAAGGAC-3′
    G6PC F, 5′-TGCAAGGGAGAACTCAGCAA-3′ R, 5′-GGACCAAGGAAGCCACAATG-3′
    PEPCK F, 5′-ACACACACACATGCTCACAC-3′ R, 5′-ATCACCGCATAGTCTCTGAA-3′
    β-Actin F, 5′-CCAGCCTTCCTTCTTGGGTAT-3′ R, 5′-TGCTGGAAGGTGGACAGTGAG-3′
    FOXP1 F, 5′-CCTCGCTCAAGGCATGATTC-3′ R, 5′-AGGACTTGGAAGGTGCCGAG-3′
    SREBP-1c F, 5′-GGAGCCATGGATTGCACATT-3′ R, 5′-GGCCCGGGAAGTCACTGT-3′
    FAS F, 5′-GTAAGTTCTGTGGCTCCAGAG-3′ R, 5′-GCCCTCCCGTACACTCACTC-3′
    ACC F, 5′-AGGAAGATGGCGTCCGCTCTG-3′ R, 5′-GGTGAGATGTGCTGGGTCAT-3′R
    CPT1a F, 5′-GAACCCCAACATCCCCAAAC-3′ R, 5′-TCCTGGCATTCTCCTGGAAT-3′
    MCAD F, 5′-AACACTTACTATGCCTCGATTGCA-3′ R, 5′-CCATAGCCTCCGAAAATCTGAA-3′
    PPARα F, 5′-ACAAGGCCTCAGGGTACCA-3′ R, 5′-GCCGAAAGAAGCCCTTACAG-3′

PCR primers used for gene and promoter amplification
    FOXP1 gene F, 5′-ATGATGCAAGAATCTGGGTCTGAG-3′; F, 5′-AGATCTCCACCATGATGCAAGAATCTG-3′ R, 5′-CCATGTCCTCATTTACTGGTTCGTC-3′; R, 5′-CTCGAGTCACTTGTCATCGTCATCCTTGTAGTCCTCCATGTCCTCAT-3′
    G6PC-922 F, 5′-ACGCGTAGACATGAGGCCAATACCAGG-3′
    G6PC-300 F, 5′-ACGCGTACTCTGTCCTGTGTCTCTGG-3′
    G6PC-133 F, 5′-ACGCGTCATCAACCTACTGGTGATGC-3′ R, 5′-AGATCTTTCCTTGGCACCTCAGGAAG-3′
    PEPCK promoter F, 5′-GATCCGGAGAAATCCCTGCCCTCA-3′ R, 5′-GATCCGAAGGGAGATCCACAGGTT-3′