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. 2015 Oct 27;290(51):30616–30623. doi: 10.1074/jbc.M115.685206

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Effect of SNAP on voltage-dependent activation and inactivation of N-type currents reconstituted with Cav2.2 mutants. A, voltage-dependent activation curves of N-type channels reconstituted with C805A or C930A, Cavβ3, and α₂δ1 subunits. Voltage-dependent activation curves were obtained by plotting the normalized conductance as a function of the command potential. B, voltage-dependent inactivation curves of N-type currents reconstituted with C805A or C930A, Cavβ3, and α₂δ1 subunits. Voltage-dependent inactivation curves were obtained using inactivation protocols. Data points were fitted using the Boltzmann equation. V_0.5 and slope factors are shown as the mean ± S.E. listed in Table 1.