FIGURE 6.

Effects of IL-1β on hepcidin and BMP2 expression in primary human hepatocytes. A, primary human hepatocytes were treated with 10 ng/ml of IL-1β for the indicated times. Total RNA was prepared and used for quantitative RT-PCR analysis of hepcidin expression. *, p = 0.01; **, p = 0.01; ***, p = 0.02. Con, control. B, the same RNA samples as in A were analyzed by quantitative RT-PCR for BMP2 expression. *, p = 0.003; **, p = 0.005. In both cases, n = 3–7 samples/group from two separate experiments.