FIGURE 2.
Analytical ultracentrifugation sedimentation equilibrium analysis of Hat1 protein complexes. Sedimentation equilibrium analytical ultracentrifugation experiments were performed at complex absorbances of 0.2, 0.5, and 0.8 Au280 nm for A–E and 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, and 0.9 Au280 nm for F, with centrifugations speeds of 6,000 rpm (blue), 9,000 rpm (red), 12,000 rpm (green), and 18,000 rpm (purple). Global fits to the data were carried out using the program heteroanalysis to generate sedimentation profiles (top panels) and the corresponding residuals (bottom panels). Molecular masses of single species complexes were determined using an ideal fitting model on the data. The model fits are shown for each centrifugation speed at one representative concentration (0.5 Au280 nm) as indicated for the following complexes: HAT-B/Asf1/H3-H4 (A), NuB4/H3-H4 (B), Hif1/H3-H4 (C), HAT-B (D), HAT-B/H3-H4 (E), and NuB4/Asf1/H3-H4 (F).