TABLE 2.
Summary of measured steady state kinetic parameters for Hat1 and complexes
ND, not determined.
| Complex | Vmax | Km | kcat | kcat/Km |
|---|---|---|---|---|
| μm/min−1 | μm | min−1 | μm−1 min−1 | |
| Substrate H3-H4 | ||||
| Hat1 | 1.59 ± 0.12 | 11.1 ± 3.2 | 15.9 ± 1.2 | 1.43 ± 0.39 |
| HAT-B | 8.56 ± 0.52 | 17.6 ± 3.3 | 85.3 ± 5.2 | 4.86 ± 1.56 |
| NuB4 | 9.44 ± 0.65 | 19.3 ± 4.0 | 94.4 ± 6.5 | 4.90 ± 1.63 |
| Substrate H4 (1–19) peptide | ||||
| Hat1 | 1.72 ± 0.16 | 275.4 ± 59.9 | 17.2 ± 1.6 | 0.063 ± 0.025 |
| HAT-B | 6.14 ± 0.19 | 87.2 ± 9.4 | 61.4 ± 1.9 | 0.71 ± 0.21 |
| NuB4 | 7.59 ± 0.24 | 82.8 ± 8.4 | 75.9 ± 2.4 | 0.92 ± 0.28 |
| Substrate Asf1/H3-H4 determined using slope | ||||
| Hat1 | ND | ND | ND | 0.47 ± 0.14 |
| HAT-B | ND | ND | ND | 2.01 ± 0.19 |
| NuB4 | ND | ND | ND | 1.75 ± 0. 21 |
| Substrate H3-H4 determined using slope | ||||
| Hat1 | ND | ND | ND | 0.52 ± 0.05 |
| HAT-B | ND | ND | ND | 2.63 ± 0.05 |
| NuB4 | ND | ND | ND | 2.39 ± 0.09 |