Fig. 1.
Serum alanine aminotransferase (ALT) and metabolic changes in Xbp1fl/fl and Xbp1−/− mice fed a high-fat/sugar (HFS) diet. A: total liver mRNA from Xbp1fl/fl and Xbp1−/− mice was analyzed by quantitative PCR (qPCR) for Xbp1 exon 2 expression (n = 4). *P < 0.001 compared with Xbp1fl/fl mice. B–F: Xbp1fl/fl and Xbp1−/− mice were fed either chow or a HFS diet for 4 or 16 wk; n ≥ 4 in each group. Serum ALT activity (B), fasting serum glucose (C), the quantitative insulin sensitivity check index (QUICKI) (D), hepatic triglyceride (TG) (E), and hepatic cholesterol (F) levels were measured or calculated. *P < 0.05 compared with chow-fed Xbp1fl/fl mice. #P < 0.05 compared with chow-fed Xbp1−/− mice. ¶P < 0.05 compared with HFS-fed Xbp1fl/fl mice.