Fig. 5.

Palmitic acid increases XBP1s expression in Huh7 cells. Huh7 cells were stably transfected with either XBP1 shRNA (Huh7-KD) or scramble shRNA (Huh7-SCR). Both groups were treated with either isopropanol (vehicle) or palmitic acid (400 μM) for 24 h. A: whole cell lysates were immunoblotted with antibody against XBP1s. GAPDH was used as a loading control. B: qPCR was performed to assess the mRNA abundance of XBP1s. The results are presented as relative expression over vehicle-treated Huh7-SCR cells. *P < 0.001 compared with vehicle-treated Huh7-SCR cells (n = 5). Vertical lines indicate reassembly of noncontiguous gel lanes.