Fig. 1.

NK2 homeobox 2 (Nkx2.2) is expressed in enteroendocrine cells in crypts and villi of the intestinal epithelium of adult mice. A: immunofluorescence of the small and large intestine of 5- to 6-wk-old Nkx2.2^LacZ/+ mice shows coexpression of Nkx2.2 with the enteroendocrine cell marker chromogranin A (Chga) in crypts and villi. Costaining of Nkx2.2 with lysozyme-expressing Paneth cells (B), Dolichos biflorus agglutinin (DBA) lectin-marked goblet cells (C), and doublecortin-like kinase 1 (Dclk1)-expressing tuft cells (D) could not be detected in the small intestine. Nkx2.2 was detected by β-galactosidase antibody staining (Nkx2.2^LacZ/+). Confocal images are shown. Magnification: ×20 (A; crypt and villus) and ×63 (B–D; insets from arrow). Dapi, 4,6-diamidino-2-phenylindole.