Fig. 7.
No change in the amount of whole Nkx2.2 lineage-traced villi after irradiation, LPS, and dextran sodium sulfate (DSS) treatment. A and B: whole-mount images of the small intestine of 7-mo-old Nkx2.2Cre/+;R26RTomato mice. A: in most mice, rare entire crypt-villus units in the small intestine are Tomato labeled (arrow). B: occasionally, the amount of entirely Tomato-stained villi increased (n = 1). C: schematic overview of the used intestinal injury models. D: quantification of Tomato-stained villi in the small intestine of 6-Gy, 12-Gy, and LPS-treated mice compared with control mice shows no significant differences (6 Gy: n = 3, 12 Gy: n = 5, LPS: n = 4, 3 “swiss” roll sections analyzed per sample). Hematoxylin and eosin stained sections of the large intestine of control (E) and DSS-treated mice (F and G) confirm that colitis is apparent 4 days after the last day of DSS treatment (F), and the large intestine is regenerated 14 days after the last day of DSS (G). Magnification: ×1 (A and B) and ×5 (E–G). H: quantification of Tomato-stained crypts in the large intestine of DSS-treated mice compared with control mice does not show a significant change in most samples. However, the large intestine of one mouse shows a dramatic increase in Nkx2.2 lineage-labeled crypts (control: n = 4, DSS: n = 10, 3 “swiss” roll sections analyzed per sample).