Fig. 5.

Effect of temperature and storage time. A: change in fluorescence intensity in glomeruli isolated and stored at room temperature (RT) compared with glomeruli isolated on ice and studied at time 0 (control). B: change in fluorescence intensity in glomeruli isolated and stored on ice for various lengths of time. All glomeruli were isolated in isotonic HBSS solution with 6% BSA. The change in fluorescence intensity in an aliquot of the stored glomeruli in response to a fall in the albumin concentration of the bath from 6 to 4% was recorded every 15 min up to 90 min after glomerular isolation. The expected change in fluorescence based on the fall in oncotic pressure of the bath is plotted in red. Values are means ± SE. Numbers in parentheses indicate the number of SD rats and glomeruli studied per group. *Significant difference (P < 0.05) compared with the change in fluorescence of glomeruli isolated on ice and studied immediately after isolation at time 0.